Variations in protein of commercial legume seeds using SDS-PAGE

نوع المستند : المقالة الأصلية

المؤلفون

Professor, Molecular Genetics. Ain Shams University, Faculty of Education, Program: Bachelor of Science and Education (preparatory and secondary), specializing in biological sciences

المستخلص

Six seeds belonging to legume species; Vicia faba, Phaseolus vulgaris and Lens culinaris; were analyzed using SDS-PAGE procedure. Extremely low polymorphism percentage (9.5%) was recorded in the protein pattern that scored a total number of 21 detectable bands with molecular weighs ranging from 170 to 39 kDa.. Only two bands with molecular weights 170 and 130 kDa disappeared in Vicia faba seeds purchased from Qalyubia. The UPGMA dendrogram confirmed the low genetic variability of the six samples that divided into two principle clusters with genetic distance of 0.25. The results led us to confirm that the quality of protein in the studied leguminous seeds was similar.

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